This second OVA series serves as an immediate prequel to the first OVA. About a hundred years after his cataclysmic battle against one of the Joestar family, the vampire Dio has been fished out of the ocean and freed. Thus begins Josef Joestar and his grandson Jotaro`s involvement in the Joestar-Dio feud as Dio`s freedom awakens the Stand-powers of Josef and street punk Jotaro for the first time. They must gather allying Stand users - warriors imbued with a Tarot card symbol that designates their power and face Dio in order to save Jotaro`s mother Holly who is affected by the curse of Dio.